當前位置:首頁 > 生理信號測量 > 大鼠.小鼠無創血壓儀MRBP
生理信號測量

大鼠.小鼠無創血壓儀MRBP

時間:2016-09-29來源:本站作者:玉研儀器

詳細介紹

大小鼠無創血壓測量系統(適合對大鼠小鼠進行無創式的血壓測量,通過對鼠尾根部的實時檢測,精確測量出大鼠、小鼠的收縮壓、舒張壓、平均壓、心率,敬請來電咨詢)


美國IITC生命科學儀器公司成立于1973年,其血壓測量系統應用于全球的生命科學研究領域,經過不斷的改進和拓展無創血壓測量系統和測痛產品系列,其產品已用于全球實驗室,也被數千篇國際論文所引用。

 

MRBP血壓測量系統能夠測量最少一只,最多同時200只動物,是經過驗證的非常有效的手段和數據采集法,可以切實協助研究人員快速準確的在最低的推薦溫度下對動物進行血壓測量。


大小鼠無創血壓測量系統的主要優勢及特點:


? 僅有的可測量從小鼠、大鼠、豚鼠到猴的血壓設備

? 采用光電傳感技術測量原理,適用于麻醉、清醒狀態下的動物血壓測量

? 內置軟件,通過觸摸操作屏控制,操作方便。也可通過電腦控制。

? 測量參數:收縮壓、舒張壓、平均壓、心率等

? 充放氣速率1-10 mmHg/秒可調

? 自動充放氣,尾套氣壓限值和測試截止壓力值均可調,提高測試效率

? 血壓測量范圍:0-300mmHg,精確度±5mmHg

? 心率測量范圍:0-1000 bpm

? 液晶屏實時顯示參數測量值和脈搏波形,并能存儲、編輯、打印數據

? USB直接儲存數據功能

? 可一次性獨立或同時測試多至200只動物

? 適用于核磁共振條件(MRI


MRBP大小鼠血壓計的主要特色:

 

無創血壓實驗需要提高受測體周圍環境的溫度。理論上,較高溫度時,測量血壓的效果很好。我們可以通過控制鼠尾的血量來控制大小鼠的體溫。當體溫高于正常值時,流經鼠尾的血液就會散掉多余的熱量。體溫越高流經鼠尾的血液就越多。研究發現,加熱大鼠的溫度高于32攝氏度、小鼠溫度高于34攝氏度時,會產生熱應力,動物的熱量就會損失一部分。這就影響了真正的血壓值(改變了差不多+/-12-28 mmHg


檢測脈沖的形式非常重要,而傳感器的選擇決定了制作工藝。壓電傳感器多年來被廣泛應用于麥克風和觸碰開關。其原理是:對傳感器施加外力后,產生電信號。但用這種傳感器作為檢測脈沖會有一系列的問題和缺陷:進行血壓測量時有較高的溫度需求(36-40℃)。

用晶體做的脈沖探測器也會出現一些問題和缺陷:進行鼠類的血壓測量時所需要的溫度高于熱力學水平溫度(36-40℃),而且加熱尾部是基于尾部脈搏是局部加熱的結果這一錯誤的假設。


1978IITC發明了光電傳感器,它將光和圖片傳感器用于脈搏的測量。當血流通過尾部,尾部血管直徑的改變導致了光量得改變,直接導致光電量的變化。IITC原件的設計(感應電子設備和咬合袖帶)不會像其他儀器一樣,會對鼠尾產生機械性的作用力,同時如果需要可以精確地切斷傳感器。光電傳感器可以在封閉的室溫下進行檢測,事實上許多使用本產品發表過的論文中,小鼠的起測溫度要低于28℃,大鼠甚至更低。


與其他的高溫法相比:

鼠類和其他動物一樣有一個內在的調節機制,我們稱為“溫度調節。正常的環境溫度大概在26-27℃,如果環境溫度高于正常溫度(28-30℃),溫度調節開始增加血液流量。這樣會導致尾部會有更多的血液流量,由于尾部是身體的一部分而并不是簡單的皮革的覆蓋,所以會自行降溫以求盡快散熱。這就是間接測量血壓的原理。

MRBP大小鼠血壓計最大的不同在于脈搏探測器上。它能在26℃或者更低的溫度下進行測量。而其他脈搏探測器由于不夠敏感就會要求更高的溫度。溫度過高會使動物死亡的風險大大增加。


大小鼠無創血壓儀的部分參考文獻:

Geraniol improves endothelial function by inhibiting NOX-2 derived oxidative stress in high fat diet fed mice

X Wang, S Zhao, M Su, L Sun, S Zhang, D Wang… - Biochemical and …, 2016 - Elsevier

... HFD controls; n = 20) for 6 weeks. After 6 weeks, systolic blood pressure was measured
using tail cuff plethysmography (IITC Life Science MRBP Blood Pressure System). The
mice were deeply anaesthetized with sodium pentobarbital ...

Disruption of calpain reduces lipotoxicity-induced cardiac injury by preventing endoplasmic reticulum stress

S Li, L Zhang, R Ni, T Cao, D Zheng, S Xiong… - … et Biophysica Acta (BBA …, 2016 - Elsevier

... Animals were acclimated in the environment for 5–10 min after occlusion cuffs and pulse
transducers (MRBP system, IITC Life Science, USA) were placed on the tail. The values of
at least 10 readings for each mouse were used for blood pressure. 2.4. ...

Influence of P2X receptors on renal medullary circulation is not altered by angiotensin II pretreatment

M Kuczeriszka, L Dobrowolski, A Walkowska… - Pharmacological …, 2016 - Elsevier

... We confirmed that with this procedure systolic blood pressure (tail-cuff method,
MRBP-3R, IITC Inc., Woodland Hills, CA, USA) increased gradually to about 190 mmHg,
compared to about 130 mmHg measured in untreated SD rats. ...

Protective Effects of At1-Receptor Blocker and Ca Antagonist Combination on Renal Function in Salt Loaded Spontaneously Hypertensive Rats/Протективни Ефекти …

K Gjorgjievska, D Zafirov, M Jurhar-Pavlova… - prilozi, 2015 - degruyter.com

... function tests. SBP was measured by a pletismografic method (IITC Life Science
MRBP Blood Pressure System, California, USA) on the rats tail. Body weight was
measured every second week of treatment. Renal functional ...

The Synthetic Tie2 Agonist Peptide Vasculotide Protects Renal Vascular Barrier Function In Experimental Acute Kidney Injury

E Rübig, J Stypmann, P Van Slyke, DJ Dumont… - Scientific …, 2016 - ncbi.nlm.nih.gov

... Immediately before the first ultrasound examination, average blood pressure and heart rate
were measured via tail cuff in each animal (5 measurements) using the mouse blood pressure
system MRBP-M01 (IITC Life science, Woodland Hills, CA, USA). ...

Changes in protein and gene expression of angiotensin II receptors (AT1 and AT2) in aorta of diabetic and hypertensive rats

R Romero-Nava, JE Rodriguez… - Clinical and …, 2016 - Taylor & Francis

... Systolic blood pressure (SBP) and heart rate were measured by a non-invasive blood pressure
tail-cuff method (MRBP Blood Pressure system, IITC, Life Science, Woodland Hills, CA) by
photoelectric detection in the rat tail and blood glucose levels were measured with a ...

Low-dose Insulin Treatment Ameliorate Glucose Metabolism in Type 1 Diabetic Rats. J Diabetes Metab 7: 635. doi: 10.4172/2155-6156.1000 635 Page 2 of 8 …

AXH Ng, SH Ton, KA Kadir - 2016 - researchgate.net

... The blood pressure of rats were measured once every week using the MRBP Mouse and Rat
Tail Cuff Method Blood Pressure System (IITC Life Sciences, USA). The tail cuff with pulse
transducer was applied onto the tail of the rat placed in a plastic restrainer. ...

Benefício da Utiliza??o do Novo Alimento Funcional Salgante sem Sódio sobre os Níveis de Press?o Arterial em Animais Hipertensos

FSM Rodrigues, MA Yasuda, L de Paula… - Revista de Pesquisa …, 2015 - pgsskroton.com.br

... Os parametros foram aferidos através de método n?o invasivo (indireto) utilizando equipamento
MRBP (Mouse Rat Blood Pressure- IITC Life Science) nos dias zero (sem tratamento com NaCl
ou Salgante - pó de aspecto cristalino, branco e fino, solúvel em água, com odor ...

 

 

Asic3 is a neuronal mechanosensor for pressure-induced vasodilation that protects against pressureulcers

B Fromy, E Lingueglia, D Sigaudo-Roussel… - Nature medicine, 2012 - nature.com

... The experimental protocol was approved by the scientific and ethics committees of the University
Claude Bernard Lyon 1, France (no. ... Blood pressure was monitored noninvasively throughout
the experiment using a tail cuff system (IITC INC/Life Science Instruments). ..

Nature Medicine,五年影響因子高達27.887

Salt–sensitive hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor

CRJ Kennedy, Y Zhang, S Brandon, Y Guan… - Nature medicine, 1999 - nature.com

... Mouse blood pressure was measured using an IITC Life Science Model 27 cuff pump with a
Model 179 blood pressure analyzer. Pressure was measured at 30 °C and was initiated as
cuffs were inflated to 200 mm Hg and deflated at a rate of 3 mm Hg/sec. ...


Chronic β-adrenergic stimulation induces myocardial proinflammatory cytokine expression

DR Murray, SD Prabhu, B Chandrasekar - Circulation, 2000 - Am Heart Assoc

... System–Audie Murphy Division, San Antonio, Tex (DRM, BC), and the University of Louisville
Health Sciences Center, Louisville ... 1 · d ?1 , n=8) or saline (n=7). After anesthesia, measures
of body weight, blood pressure, and heart rate (tail cuff, IITC Life Science model 29 ...

 

Circulation 最新的影響因子為15.202 (2013) ,該雜志 2011年在 CARDIAC & CARDIOVASCULAR SYSTEMS (心血管) 同類期刊中的影響因子排名第 1位。

Endogenous Ligand of α1 Sodium Pump, Marinobufagenin, Is a Novel Mediator of Sodium Chloride–Dependent Hypertension

OV Fedorova, MI Talan, NI Agalakova, EG Lakatta… - Circulation, 2002 - Am Heart Assoc

... Systolic BP (SBP) was measured weekly by tail-cuff plethysmography (IITC model 31, IITC Life
Science). ... 1969; 1 (suppl 24, 25): 21–33. ? Blaustein MP. Sodium ions, calcium ions, blood
pressure regulation and hypertension: a reassessment and a hypothesis. Am J Physiol. ...

 

    您想了解更多詳細資料嗎?

    請與我們聯系:

    TEL021-35183767,021-54377179

        18502129044

    QQ3007536034

    微信:yuyanbio

qq在線客服